The graph will show a steadily rising line, turning into a plateau, after reaching a certain point. Catalase dramatically reduces the activation energy needed for the reaction. If you need this or any other sample, we can send it to you via email. A stopwatch or any similar device can be used to monitor the time of reaction. Since the catalysts boost up the speed of the reaction, the reaction rate will go up when there is more catalysts, drawing exponential graph. Place yeast in the conical flask using a spatula until you have reached the right weight 0.
However, if we had more trials with broader concentration of catalase, the graph will definitely going to be a form of plateau graph. Limitations are individual activity within the classroom which can change the temperature and pressure in the room. Temperature directly affects the shape of the active site. If you are the original author of this content and no longer wish to have it published on our website then please click on the link below to request removal:. Some articles have Google Maps embedded in them.
I am experienced in the fields of biology, physics and chemistry, and I enjoy writing on these topics. In this case, the enzyme I will use in the investigation is catalase from celery extract, which is concluding as hydrolytic enzyme.
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Catalase is an enzyme found in living cells which catalyzes the decomposition of hydrogen peroxide into water and oxygen. The dependent variable the one I intend to measure is the volume of gas produced in each reaction. All the reactions must be carried out for a standard amount of time. Conduct a pilot study to check acceptable concentrations of hydrogen peroxide for the investigation by measuring the quantity of oxygen evolved from a reaction in which a low concentration of hydrogen peroxide is treated with 3 parts lesser quantity of the enzyme Catalase.
This is shown when oxygen stops being produced and the same results are recorded five times. The steadily raising line in the beginning of the curve plotted caatlase the increased rate of catalysis by the enzyme Catalase.
Size Gcse Coursework Biology Potato Catalase Hydrogen Peroxide
A substrate is the molecule, which can bind into the active of enzyme. This is used to provide traffic data and reports to the authors of articles on the HubPages Service.
Record the quantity of oxygen evolved and during a standard time. This heat energy is transferred to the peoxide.
We will write a custom paper sample on How different concentration of enzyme catalase affects the rate of breaking down substrate hydrogen peroxide specifically for you.
Analysis From peroide graph, I can see that as the concentration of hydrogen peroxide decreased, the volume of oxygen produced decreased as a direct result.
Effect of Substrate Concentration on the Rate of Activity of Catalase | Owlcation
I will use the following concentrations of hydrogen peroxide: As it alters its peroxid, the enzyme puts a strain on the substrate molecule and thereby lowers its activation energy.
IB Biology on decomposition of Hydrogen Peroxide by enzyme I will not hydroten any substance except celery solution and hydrogen peroxide during the experiment. Free potato catalase Essays and Papers – helpme. Click to learn more https: In addition, the rate of decomposition did not stop increasing and did not stay constant with increasing catalase concentration.
Surface Area of Yeast Molecules I ground up the yeast to try to make the surface area as similar as possible because surface area is a major factor in my experiment.
Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time. So to avoid this problem, I will keep the water bath has the temperature of 25?
This is used to prevent bots and spam. The volume of hydrogen peroxide was kept constant at 5 mL in all test tubes while the volume of water and grinded potatoes were adjusted to manipulate the concentration of catalase in the mixture.
I have drawn a graph based on these average results with a curve of best fit for each concentration that will allow me to identify any anomalies.
Method of measuring dependant variable 1. Also, in my main procedure, I will grind a larger coursedork of yeast more than I needand then weigh it, rather than weighing catalade yeast and then grinding it. Therefore if the concentration of enzyme is increased, the number of collision with the substrate molecule and hence the rate of reaction will increase.
If my replicates had not coursewodk so close I would have had to change the tube. Prepare 6 different concentrations of hydrogen peroxide in gradual increments, taking the standard low concentration of corsework peroxide used in the pilot study as defined in STEP B, POINT 1 as the lowest concentration in the gradually increasing scale.