If 11A infection is capable of producing an O-acetylation specific response, we expect sera from 11A infected mice to preferably bind and opsonize 11A PS and bacteria. All sections are critical and need to show a complete picture. The candidate has described their training program very well and their models can be refined experimentally by their collaborators. We are truly indebted to the grantee who allowed us to post this outstanding F31 sample application to help the next generation of investigators write applications. Sponsors, Collaborators, and Consultants:
This process was capable to induce antibodies that are specific for 11A PS and not 11E PS 32 , so we assume that O-acetylation is at least partially conserved in the immunization product. Identification of natural pneumococcal isolates expressing serotype 6D by genetic, biochemical and serological characterization. Unfortunately, my research was interrupted by Hurricane Katrina, and I was unable to obtain enough data for publication. Already has 1st author publication. Timeline for the F31 application Start early. Strengths Particularly good statement of goals — believable, enthusiastic, sincere.
To facilitate the study of serotypes that readily seroswitch, we must develop methods that can detect and distinguish clones expressing closely related serotypes within a culture. In some cases, protective antibody will be given passively. Identification of natural pneumococcal isolates expressing serotype 6D by genetic, biochemical and serological characterization. Despite the large diversity of capsule structure, only a few prevalent serotypes are responsible for most of pneumococcal disease 7.
O-acetyl groups are susceptible to mild hydrolysis and other factors, such as storage time, so the degree of O-acetylation of 11A may have decreased during preparation, storage and administration of the vaccine.
The rest is personal. After incubation, surviving bacteria will be spotted on plates containing antibiotic selective nrxa, so that surviving 11Astr CFU will be enumerated on streptomycin plates, and 11Ekan CFU on kanamycin plates. By genotyping bugs collected from different sites and implementing population genetics models, we ztatement the existence of barriers to intermigration by bug populations. January 29, Minutes: This leads to your letter writers not being able to submit their letters of recommendation ask me how I know….
However, small capsule expression differences will have minimal effect on antibodymediated opsonophagocytosis. This way you can correlate specific feedback with the actual context.
NIH F31 fellowship Do’s and Don’ts – ChemToolman
Thus, if you are in a big lab even a big name lab it might be good to find a co-sponsor who has a smaller lab with a good history of mentoring. This course is also required for all graduate students trained in the biomedical sciences. Understanding how to anticipate ways that dtatement may adapt to immune response is important in the design of effective vaccines. A new pneumococcal serotype, 11E, has variably inactivated wcjE gene.
Which you start with really depends on how well defined your thesis project is. I think it is important for potential applicants of the F31 fellowship to get a look at what reviewers are saying about successful applications and ones they turned down. Lipoteichoic acid is important in innate immune responses to grampositive bacteria. Next, we will transform the streptomycin resistant strain with a Janus cassette containing a kanamycin resistance gene and a streptomycin susceptibility gene, to disrupt wcjE, as previously described 5.
In developing this study plan and application, I have already gained a better understanding of grant writing. This is a special instance that an unusually large group of investigators focus on a same pathogen, and this makes for an ideal environment for me to address the hypothesis of this proposal.
Your sponsor and co-sponsor will then need to state how many people they will be training during your fellowship.
Serological and structural changes are attributed to disruption of the capsule synthesis gene wcjE in all 11E strains, indicating that the gene encodes an O-acetyltransferase OAcT 5.
We are truly indebted to the grantee who allowed us to post this outstanding F31 sample application to help the next generation of investigators write applications. It was that indecision that the reviewers saw as a negative in their application.
Jurgen Brojatsch studying the kinetics of anthrax-toxin mediated killing of macrophages. However, serological assays that make use of more antigenically nsra monoclonal antibodies mAb have been developed and have led to the identification of new serotypes 2, 4, 8.
Calix, Sample F31 Application and Summary Statement
If 11A infection is capable of producing an O-acetylation specific response, we expect sera from 11A infected mice to preferably bind and opsonize 11A PS and bacteria. It is these experiences that have prepared me for achieving the goals stated in this proposal.
The laboratory is productive with publications of both clinical and basic science relevance. Source is not described and justification for exemption is not provided. Murine models of pneumococcal infection have been well established nra, Mentally prepare for some late night writing after a day in the lab.
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